Artículo Científico presentado por Diana Paulina Sucar Escamilla []

Miembro del Programa de Honores. Licenciatura en Ingeniería Química. Departamento de Ingeniería Química, Alimentos y Ambiental. Escuela de Ingeniería, Universidad de las Américas Puebla.

Jurado Calificador

Presidente: Dr. Aurelio López Malo Vigil
Vocal y Director: Dr. Enrique Palou García
Secretario: Dra. Fátima Reyes Jurado

Cholula, Puebla, México a 4 de diciembre de 2018.


The present study investigated the effect of a sequential treatment of high intensity light pulses (HILP) followed by lemongrass essential oil (EO) in vapor phase to inactivate Salmonella Typhimurium on sunflower seeds. First, the susceptibility of S. Typhimurium to HILP was evaluated in-vitro. Initial microbial load was inactivated with a treatment time of 8 s with a corresponding fluence of 10.80 J/cm2 and a reduction of 10 log cycles. Then, the in-vivo effect of HILP on inoculated sunflower seeds was evaluated. A reduction of 3 log cycles was obtained with a treatment of 12 s; for larger treatments, the microbial load remained constant. The inactivation curves for both in-vitro and in-vivo tests were adequately fitted using the Weibull equation. Furthermore, surviving populations of S. Typhimurium on inoculated sunflower seeds were then exposed to an additional treatment with lemongrass EO in vapor phase. Studied microbial load was inactivated with a concentration of 294.12 mL EO/Lair, corresponding to a reduction of 3 log cycles. Thus, it was demonstrated that the HILP treatment in sequential association to vapor phase lemongrass EO increased the inactivation of S. Typhimurium on studied seeds. Further research is needed to evaluate the effect of each tested technology and its combination on sensory parameters of sunflower seeds.

Keywords: Salmonella Typhimurium; sunflower seed; high intensity light pulses; vapor phase essential oil; Weibull equation

Table of content



Capítulo 1. Introduction

Capítulo 2. Materials and Methods

Capítulo 3. Results and Discussion

Capítulo 4. Conclusions



Sucar Escamilla, D. P. 2018. Inactivation of Salmonella Typhimurium on Sunflower Seeds by High Intensity Light Pulses and Lemongrass Essential Oil in Vapor Phase. Artículo Científico Licenciatura. Ingeniería Química. Departamento de Ingeniería Química, Alimentos y Ambiental, Escuela de Ingeniería, Universidad de las Américas Puebla. Diciembre. Derechos Reservados © 2018.