Resumen

In the present work, citrate-stabilized spherical gold nanoparticles (AuNPs) with different diameters were synthesized by HAuCl4 reduction. Physical and chemical properties of the gold nanoparticles were characterized through dynamic light scattering (DLS), scanning electron microscopy (SEM) and ultraviolet-visible (UV-vis) spectroscopy. Bioconjugation of the nanoparticles was carried out to create an antibody-AuNPs colorimetric biosensor for the detection of NS1 protein, present in the patient´s blood during dengue virus infection. Antibody bonding to the surface of the AuNPs was accomplished through oriented and non-oriented adsorption to compare their sensing capabilities. The antibody-AuNPs conjugates were exposed to dengue virus infected serums presenting a shift in their absorbance spectrum.

Palabras clave: Nanotechnology, Nanosensors, Gold Nanoparticles, Dengue virus.

El acceso a esta tesis es restringido.

Figueroa González, A. 2019. Synthesis and Characterization of Gold Nanoparticles as a Platform for Dengue Virus Detection. Tesis Licenciatura. Nanotecnología e Ingeniería Molecular. Departamento de Ciencias Químico Biológicas, Escuela de Ciencias, Universidad de las Américas Puebla. Mayo. Derechos Reservados © 2019.